Center for Molecular Medicine
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Division of Chemistry and Chemical Engineering
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Center for Molecular Medicine
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Division of Chemistry and Chemical Engineering
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Center for Molecular Medicine
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Electron Paramagnetic Resonance (EPR) Facility
*
Glass Shop
*
Liquid NMR Facility
*
Machine Shop
*
Mass Spectrometry
*
Solid State NMR Facility
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FY2023 CCE Rate Sheet
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For Postdoctoral Scholars
News & Events
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News
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CCE Newsletters
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Biochemistry
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Chemical Engineering
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Chemical Physics
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Inorganic Chemistry
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Organic Chemistry
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PhD Thesis Defense
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James LuValle Award
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Day of Inclusion 2025
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Center for Molecular Medicine
Center for Molecular Medicine
Dedicated to understanding the intricacies of cellular signaling, the Center for Molecular Medicine was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation.
If use of the Center instrumentation has contributed significantly to your research, we request that you cite the Center in the acknowledgments section of your publication. For example: "This work was supported by…, and by the Center for Molecular Medicine at California Institute of Technology."
Access to CM2 Instruments
* Please contact the appropriate caretakers for each instrument you would like to use for the instrument training.
* Upon completion of training, contact (to be announced) to get access.
People in the Facility
Faculty members: Dr. Dennis Dougherty, Dr. Jacqueline Barton, Dr. Peter Dervan, Dr. Linda Hsieh-Wilson, Dr. Shu-ou Shan, Dr. Mikhail Shapiro
Safety Officer: Trevor Fochtman
Lab Assistants: Please see the table below for instrument caretakers
Seminars
Every Thursday
12:00 - 1:00 PM
151 Crellin
Instrumentation
Crellin Room 262 (Bacterial)
Flexstation 3
Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents.
Contact: John O. Chapman, , [email protected]
Odyssey Li-Cor IR Imager
Quantitative scanning of western blots at 700 and 800 nm.
Contact: Trevor Fochtman, [email protected]
Beckman LS 6000SC Scintillation Counter
Image Lightbox
Download Full Image
The scintillation counter is designed to provide highly accurate, automated counting of the level of radioactivity in radioactivity-tagged samples. Instrument used to count radioactive samples. Note: New users must complete Radioactivity Safety Training with Haick Issaian ( [email protected] ), the Institute Radiation Safety Officer, before getting access to the scintillation counte.
Contact: Haick Issaian [email protected]
Nanodrop 2000
Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL.
Contact: Li-En Lin, [email protected]
QIAcube
Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins.
Contact:
Innova 43 Incubator Shaker
Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C.
Contact: Trevor Fochtman, [email protected]
Avanti J-26 Centrifuge
Centrifuge up to 6 1-Liter cultures.
Contact: Alex Siegel, [email protected]
Ultrospec 10
Portable spectrophotometer for OD600 readings.
Contact: Trevor Fochtman, [email protected]
-80°C freezer
Intended for use as a back-up of individual lab freezers.
Contact: Trevor Fochtman, [email protected]
Zeta Potential Analyzer (DLS instrument)
Image Lightbox
Download Full Image
Contact: Trevor Fochtman, [email protected]
Tabletop microcentrifuge
Contact: Trevor Fochtman, [email protected]
Crellin Room 260 (Mammalian)
Typhoon FLA 9000
High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software.
Contact: Maria Altshuller, [email protected]
Amaxa 4D-Nucleofector
Efficient electroporation of mammalian cells for transfection.
Contact: James Linton, [email protected]
CFX 96 Real-Time PCR Detection System
Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA.
Contact: Boyoung Shin [email protected] and Tom Sidwell [email protected]
Neon Transfection System
High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes.
Contact:
Nikon SMZ1000 Microscope
Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space.
Contact:
Nikon TS100 Microscope
Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells.
Contact:
Clean Bench
Provides sterile environment for sensitive dissections or microscope work.
Laminar Flow Hoods/Biosafety Cabinets
Provides sterile environment for mammalian cell work.
Mammalian Tissue Culture CO2 Incubator
Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells.
Eppendorf Centrifuge 5702
Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes.
Contact: Trevor Fochtman, [email protected]
Refrigerator
Contact: Trevor Fochtman, [email protected]
Tabletop microcentrifuge
Contact: Trevor Fochtman, [email protected]
Water bath
Contact: Trevor Fochtman, [email protected]
Instrumentation Caretakers
For training or troubleshooting, please contact the appropriate caretakers.
Equipment
Caretaker
Contact info
Biosafety cabinet (TC hood)
Chemical fume hood
Trevor Fochtman
[email protected]
Amaxa 4-D Nucleofector
James Linton
[email protected]
Benchtop centrifuges
Trevor Fochtman
[email protected]
Benchtop water bath
MilliQ water deionizer
Trevor Fochtman
[email protected]
Typhoon FLA 9000 gel imager
Maria Altshuller
[email protected]
Inverted fluorescence microscope (Nikon TS100)
Upright dissection microscope (Nikon SMZ1000) + Clean bench
Affinity ITC
Chia-Yu Chien
[email protected]
Mammalian cell incubator
Zeta Potential Analyzer
Ernesto Criado Hidalgo & Di Wu
[email protected] , [email protected]
BioRad qPCR (CFX 96 RT PCR)
Boyoung Shin and Tom Sidwell
[email protected] , [email protected]
4°C refrigerator
FlexStation microplate reader
John O. Chapman
[email protected]
Beckman Avanti J-26 floor centrifuge
Alex Siegel
[email protected]
-80°C freezer
Julio Revilla
[email protected]
Nanodrop 2000
Li-En Lin
[email protected]
Li-Cor IR imager
Ruilin Qian
[email protected]
Beckman Scintillation Counter
Haick
Issaian
[email protected]
Shaking incubator for bacteria
Trevor Fochtman
[email protected]
Ultrospec cell density meter
QIAcube
Neon transfection system
Ice maker
Mailing address
Caltech Center for Molecular Medicine
Division of Chemistry and Chemical Engineering
MC 164-30
California Institute of Technology
Pasadena, CA 91125
USA
California Institute of Technology
1200 East California Boulevard
Pasadena, California 91125
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- Electron Paramagnetic Resonance (EPR) Facility [direct]
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